Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability

Improved thermostability of Clostridium thermocellum 1 endoglucanase Cel 8 A using consensus - guided mutagenesis 2 3

3 Michael Anbar, Ozgur Gul, Raphael Lamed, Ugur O. Sezerman 4 and Edward A. Bayer* 5 6 Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 7 76100 Israel 8 2 Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, 9 Istanbul, Turkey 10 3 Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 11 Ramat Aviv 69978 Israel...

ethanol - tolerant strain of Clostridium phytofermentans

Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: heterologous expression, characterization, and synergy with family 48 cellobiohydrolase.

The glycoside hydrolase family 9 cellulase (Cel9) from Clostridium phytofermentans has a multi-modular structure and is essential for cellulose hydrolysis. In order to facilitate production and purification of Cel9, recombinant Cel9 was functionally expressed in Escherichia coli. Cel9 exhibited maximum activity at pH 6.5 and 65 degrees C on carboxymethyl cellulose in a 10-min reaction period. T...

Characterization of Three -Galactoside Phosphorylases from Clostridium phytofermentans

We characterized three D-galactosyl133-N-acetyl-Dhexosamine phosphorylase (EC 2.4.1.211) homologs from Clostridium phytofermentans (Cphy0577, Cphy1920, and Cphy3030 proteins). Cphy0577 and Cphy3030 proteins exhibited similar activity on galacto-N-biose (GNB; D-Gal133-D-GalNAc) and lacto-N-biose I (LNB; D-Gal133-DGlcNAc), thus indicating that they are D-galactosyl133-Nacetyl-D-hexosamine phospho...

Characterization of endoglucanase A from Clostridium cellulolyticum.

A construction was carried out to obtain a high level of expression in Escherichia coli of the gene celCCA, coding for the endoglucanase A from Clostridium cellulolyticum (EGCCA). The enzyme was purified in two forms with different molecular weights, 51,000 and 44,000. The smaller protein was probably the result of proteolysis, although great care was taken to prevent this process from occurrin...